› 1k88

X-ray diffraction
2.7Å resolution

Crystal structure of procaspase-7

Released:
DOI: 10.2210/pdb1k88/pdb
PDB entry 1k88 coloured by chain, front view.
PDB entry 1k88 coloured by chain, side view.
PDB entry 1k88 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a procaspase-7 zymogen: mechanisms of activation and substrate binding.
Chai J, Wu Q, Shiozaki E, Srinivasula SM, Alnemri ES, Shi Y
Cell 107 399-407 (2001)
PMID: 11701129

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Caspase-7 subunit p20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55210 (Residues: 51-303; Coverage: 84%)
Gene names: CASP7, MCH3
Sequence domains: Caspase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 91.2Å b: 91.2Å c: 185.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.227 0.253
Expression system: Escherichia coli

Quick links

Citations

41 review citations

Molecular mechanisms of caspase regulation during apoptosis.
Riedl et al. (2004)
40 more

9 mentions without citation

Caspase-7: a protease involved in apoptosis and inflammation.
Lamkanfi et al. (2010)
8 more