› 1ken

X-ray diffraction
3.5Å resolution

INFLUENZA VIRUS HEMAGGLUTININ COMPLEXED WITH AN ANTIBODY THAT PREVENTS THE HEMAGGLUTININ LOW PH FUSOGENIC TRANSITION

Released:
DOI: 10.2210/pdb1ken/pdb
PDB entry 1ken coloured by chain, front view.
PDB entry 1ken coloured by chain, side view.
PDB entry 1ken coloured by chain, top view.
Source organisms:
Primary publication:
An antibody that prevents the hemagglutinin low pH fusogenic transition.
Barbey-Martin C, Gigant B, Bizebard T, Calder LJ, Wharton SA, Skehel JJ, Knossow M
Virology 294 70-4 (2002)
PMID: 11886266

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-134701 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 328 amino acids
Theoretical weight: 36.07 KDa
Source organism: Influenza A virus (A/X-31(H3N2))
UniProt:
  • Canonical: P03438 (Residues: 17-344; Coverage: 60%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Hemagglutinin HA2 chain Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 175 amino acids
Theoretical weight: 20.21 KDa
Source organism: Influenza A virus (A/X-31(H3N2))
UniProt:
  • Canonical: P03437 (Residues: 346-520; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Immunoglobulin kappa constant Chains: L, U
Molecule details ›
Chains: L, U
Length: 213 amino acids
Theoretical weight: 23.28 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-105; Coverage: 98%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-1 chain C region, membrane-bound form Chains: H, T
Molecule details ›
Chains: H, T
Length: 221 amino acids
Theoretical weight: 24.35 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01869 (Residues: 1-101; Coverage: 26%)
Gene names: Igh-4, Ighg1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 143.041Å b: 315.588Å c: 97.029Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.323

Quick links

Citations

20 review citations

The many mechanisms of viral membrane fusion proteins.
Earp et al. (2005)
19 more

42 mentions without citation

Playing hide and seek: how glycosylation of the influenza virus hemagglutinin can modulate the immune response to infection.
Tate et al. (2014)
41 more