› 1ki1

X-ray diffraction
2.3Å resolution

Guanine Nucleotide Exchange Region of Intersectin in Complex with Cdc42

Released:
DOI: 10.2210/pdb1ki1/pdb
PDB entry 1ki1 coloured by chain, front view.
PDB entry 1ki1 coloured by chain, side view.
PDB entry 1ki1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors.
Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J
Nat Struct Biol 9 468-75 (2002)
PMID: 12006984

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158136 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cell division control protein 42 homolog Chains: A, C
Molecule details ›
Chains: A, C
Length: 188 amino acids
Theoretical weight: 20.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60953 (Residues: 1-188; Coverage: 98%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Intersectin-1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 352 amino acids
Theoretical weight: 40.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15811 (Residues: 1229-1581; Coverage: 21%)
Gene names: ITSN, ITSN1, SH3D1A
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 85.37Å b: 79.23Å c: 116.41Å
α: 90° β: 111.51° γ: 90°
R-values:
R R work R free
0.231 0.231 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors.
Rossman et al. (2005)
21 more

21 mentions without citation

Always look on the bright site of Rho: structural implications for a conserved intermolecular interface.
Dvorsky et al. (2004)
20 more