› 1klg

X-ray diffraction
2.4Å resolution

Crystal structure of HLA-DR1/TPI(23-37, Thr28-->Ile mutant) complexed with staphylococcal enterotoxin C3 variant 3B2 (SEC3-3B2)

Released:
DOI: 10.2210/pdb1klg/pdb
PDB entry 1klg coloured by chain, front view.
PDB entry 1klg coloured by chain, side view.
PDB entry 1klg coloured by chain, top view.
Source organisms:
Primary publication:
Minor structural changes in a mutated human melanoma antigen correspond to dramatically enhanced stimulation of a CD4+ tumor-infiltrating lymphocyte line.
Sundberg EJ, Sawicki MW, Southwood S, Andersen PS, Sette A, Mariuzza RA
J Mol Biol 319 449-61 (2002)
PMID: 12051920

Function and Biology Details

Reactions catalysed: 
Glycerone phosphate = methylglyoxal + phosphate
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-135328 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 20.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 29-205; Coverage: 77%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01911 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Triosephosphate isomerase Chain: C
Molecule details ›
Chain: C
Length: 15 amino acids
Theoretical weight: 1.48 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P60174 (Residues: 23-37; Coverage: 6%)
Gene names: TPI, TPI1
Enterotoxin type C-3 Chain: D
Molecule details ›
Chain: D
Length: 239 amino acids
Theoretical weight: 27.72 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0L5 (Residues: 28-266; Coverage: 100%)
Gene name: entC3
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: R3
Unit cell:
a: 172.986Å b: 172.986Å c: 121.435Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Interplay between superantigens and immunoreceptors.
Petersson et al. (2004)
2 more

19 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
18 more