› 1klq

Solution NMR

The Mad2 Spindle Checkpoint Protein Undergoes Similar Major Conformational Changes upon Binding to Either Mad1 or Cdc20

Released:
DOI: 10.2210/pdb1klq/pdb
PDB entry 1klq coloured by chain, front view.
PDB entry 1klq coloured by chain, side view.
PDB entry 1klq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20.
Luo X, Tang Z, Rizo J, Yu H
Mol Cell 9 59-71 (2002)
PMID: 11804586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162283 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2A Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13257 (Residues: 11-205; Coverage: 95%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain
Mad2-binding peptide Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

52 review citations

The spindle-assembly checkpoint in space and time.
Musacchio et al. (2007)
51 more

10 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
9 more