› 1ktl

X-ray diffraction
3.1Å resolution

The human non-classical major histocompatibility complex molecule HLA-E

Released:
DOI: 10.2210/pdb1ktl/pdb
PDB entry 1ktl coloured by chain, front view.
PDB entry 1ktl coloured by chain, side view.
PDB entry 1ktl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities.
Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE
J Biol Chem 278 5082-90 (2003)
PMID: 12411439

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dodecamer
PDBe Complex ID:
PDB-CPX-146754 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, C
Molecule details ›
Chains: A, C
Length: 274 amino acids
Theoretical weight: 31.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 22-295; Coverage: 81%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Peptide VTAPRTLLL Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 984 Da

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 178.4Å b: 178.4Å c: 87.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.238 0.28
Expression system: Escherichia coli

Quick links

Citations

48 review citations

MHC class Ib molecules bridge innate and acquired immunity.
Rodgers et al. (2005)
47 more

4 mentions without citation

Single-Nucleotide Polymorphisms (SNP) Mining and Their Effect on the Tridimensional Protein Structure Prediction in a Set of Immunity-Related Expressed Sequence Tags (EST) in Atlantic Salmon (Salmo salar).
Vallejos-Vidal et al. (2019)
3 more