› 1kvo

X-ray diffraction
2Å resolution

HUMAN PHOSPHOLIPASE A2 COMPLEXED WITH A HIGHLY POTENT SUBSTRATE ANOLOGUE

Released:
DOI: 10.2210/pdb1kvo/pdb
PDB entry 1kvo coloured by chain, front view.
PDB entry 1kvo coloured by chain, side view.
PDB entry 1kvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution X-ray crystallography reveals precise binding interactions between human nonpancreatic secreted phospholipase A2 and a highly potent inhibitor (FPL67047XX).
Cha SS, Lee D, Adams J, Kurdyla JT, Jones CS, Marshall LA, Bolognese B, Abdel-Meguid SS, Oh BH
J Med Chem 39 3878-81 (1996)
PMID: 8831753

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, membrane associated Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 124 amino acids
Theoretical weight: 13.95 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14555 (Residues: 21-144; Coverage: 100%)
Gene names: PLA2B, PLA2G2A, PLA2L, RASF-A
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 12 x CA
Ligand OAP 6 x OAP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 64.62Å b: 114.44Å c: 64.71Å
α: 90° β: 119.9° γ: 90°
R-values:
R R work R free
0.201 0.201 0.273
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention.
Dennis et al. (2011)
1 more

12 mentions without citation

Pharmacophore-based similarity scoring for DOCK.
Jiang et al. (2015)
11 more