› 1l9l

X-ray diffraction
0.92Å resolution

GRANULYSIN FROM HUMAN CYTOLYTIC T LYMPHOCYTES

Released:
DOI: 10.2210/pdb1l9l/pdb
PDB entry 1l9l coloured by chain, front view.
PDB entry 1l9l coloured by chain, side view.
PDB entry 1l9l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Granulysin crystal structure and a structure-derived lytic mechanism.
Anderson DH, Sawaya MR, Cascio D, Ernst W, Modlin R, Krensky A, Eisenberg D
J Mol Biol 325 355-65 (2003)
PMID: 12488100

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granulysin Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22749 (Residues: 63-136; Coverage: 60%)
Gene names: GNLY, LAG2, NKG5, TLA519
Sequence domains: Saposin-like type B, region 2
Structure domains: Saposin-like

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand MPO 1 x MPO
Ligand EOH 3 x EOH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 23.265Å b: 60.436Å c: 23.591Å
α: 90° β: 90.99° γ: 90°
R-values:
R R work R free
0.138 0.137 0.19
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal.
Lieberman J. (2003)
29 more

25 mentions without citation

A short guided tour through functional and structural features of saposin-like proteins.
Bruhn H. (2005)
24 more