› 1lk6

X-ray diffraction
2.8Å resolution

Structure of dimeric antithrombin complexed with a P14-P9 reactive loop peptide and an exogenous tripeptide

Released:
DOI: 10.2210/pdb1lk6/pdb
PDB entry 1lk6 coloured by chain, front view.
PDB entry 1lk6 coloured by chain, side view.
PDB entry 1lk6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Serpin polymerization is prevented by a hydrogen bond network that is centered on his-334 and stabilized by glycerol.
Zhou A, Stein PE, Huntington JA, Carrell RW
J Biol Chem 278 15116-22 (2003)
PMID: 12578831

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133782 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antithrombin-III Chains: I, L
Molecule details ›
Chains: I, L
Length: 432 amino acids
Theoretical weight: 49.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01008 (Residues: 33-464; Coverage: 100%)
Gene names: AT3, PRO0309, SERPINC1
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:
antithrombin P14-P9 peptide Chain: C
Molecule details ›
Chain: C
Length: 7 amino acids
Theoretical weight: 561 Da
Source organism: Homo sapiens
Expression system: Not provided
exogenous tripeptide formyl-MLF Chain: D
Molecule details ›
Chain: D
Length: 3 amino acids
Theoretical weight: 438 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand NDG 6 x NDG
Ligand NAG 2 x NAG
Ligand GOL 1 x GOL
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21
Unit cell:
a: 68.811Å b: 99.908Å c: 87.292Å
α: 90° β: 104.44° γ: 90°
R-values:
R R work R free
0.202 0.202 0.266
Expression system: Not provided

Quick links

Citations

5 review citations

Conformational pathology of the serpins: themes, variations, and therapeutic strategies.
Gooptu et al. (2009)
4 more

1 mention without citation

Exploring functional roles of multibinding protein interfaces.
Tyagi et al. (2009)