› 1lot

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX OF ACTIN WITH VITAMIN D-BINDING PROTEIN

Released:
DOI: 10.2210/pdb1lot/pdb
PDB entry 1lot coloured by chain, front view.
PDB entry 1lot coloured by chain, side view.
PDB entry 1lot coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the complex between actin and human vitamin D-binding protein at 2.5 A resolution.
Head JF, Swamy N, Ray R
Biochemistry 41 9015-20 (2002)
PMID: 12119014

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136196 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vitamin D-binding protein Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P02774 (Residues: 17-474; Coverage: 100%)
Gene name: GC
Sequence domains:
Structure domains: Serum Albumin; Chain A, Domain 1
Actin, alpha skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 6 x GOL
Ligand CA 1 x CA
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 80.171Å b: 87.341Å c: 159.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.279

Quick links

Citations

7 review citations

Vitamin D Binding Protein, Total and Free Vitamin D Levels in Different Physiological and Pathophysiological Conditions.
Bikle et al. (2019)
6 more

4 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
3 more