› 1lto

X-ray diffraction
2.2Å resolution

Human alpha1-tryptase

Released:
DOI: 10.2210/pdb1lto/pdb
PDB entry 1lto coloured by chain, front view.
PDB entry 1lto coloured by chain, side view.
PDB entry 1lto coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human alpha1-tryptase reveals a blocked substrate-binding region.
Marquardt U, Zettl F, Huber R, Bode W, Sommerhoff C
J Mol Biol 321 491-502 (2002)
PMID: 12162961

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-, but with more restricted specificity than trypsin.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172371 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptase alpha/beta-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 245 amino acids
Theoretical weight: 27.73 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q15661 (Residues: 31-275; Coverage: 95%)
Gene names: TPS1, TPS2, TPSAB1, TPSB1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 91.441Å b: 110.685Å c: 130.092Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.267
Expression system: Komagataella pastoris

Quick links

Citations

9 review citations

Diagnostic value of tryptase in anaphylaxis and mastocytosis.
Schwartz LB. (2006)
8 more

6 mentions without citation

Serine proteases.
Di Cera E. (2009)
5 more