› 1m1u

X-ray diffraction
2.3Å resolution

AN ISOLEUCINE-BASED ALLOSTERIC SWITCH CONTROLS AFFINITY AND SHAPE SHIFTING IN INTEGRIN CD11B A-DOMAIN

Released:
DOI: 10.2210/pdb1m1u/pdb
PDB entry 1m1u coloured by chain, front view.
PDB entry 1m1u coloured by chain, side view.
PDB entry 1m1u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An isoleucine-based allosteric switch controls affinity and shape shifting in integrin CD11b A-domain.
Xiong JP, Li R, Essafi M, Stehle T, Arnaout MA
J Biol Chem 275 38762-7 (2000)
PMID: 11034990

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrin alpha-M Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11215 (Residues: 139-331; Coverage: 17%)
Gene names: CD11B, CR3A, ITGAM
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P43
Unit cell:
a: 45.7Å b: 45.7Å c: 94.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.248
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Integrins: bidirectional, allosteric signaling machines.
Hynes RO. (2002)
20 more

4 mentions without citation

CD11b agonists offer a novel approach for treating lupus nephritis.
Villanueva et al. (2022)
3 more