› 1m5o

X-ray diffraction
2.2Å resolution

Transition State Stabilization by a Catalytic RNA

Released:
DOI: 10.2210/pdb1m5o/pdb
PDB entry 1m5o coloured by chain, front view.
PDB entry 1m5o coloured by chain, side view.
PDB entry 1m5o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Transition state stabilization by a catalytic RNA.
Rupert PB, Massey AP, Sigurdsson ST, Ferré-D'Amaré AR
Science 298 1421-4 (2002)
PMID: 12376595

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140460 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
U1 small nuclear ribonucleoprotein A Chains: C, F
Molecule details ›
Chains: C, F
Length: 100 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 1-100; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA SUBSTRATE Chains: A, D
Molecule details ›
Chains: A, D
Length: 21 nucleotides
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
RNA HAIRPIN RIBOZYME Chains: B, E
Molecule details ›
Chains: B, E
Length: 92 nucleotides
Theoretical weight: 29.81 KDa
Source organism: Homo sapiens
Expression system: Not provided
Sequence domains: Hairpin ribozyme 1 from viruses-like metatranscriptomes

Ligands and Environments

1 bound ligand:
Ligand CA 37 x CA
1 modified residue:
Ligand AVC 2 x AVC

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 259.38Å b: 44.22Å c: 102.5Å
α: 90° β: 106.3° γ: 90°
R-values:
R R work R free
0.229 0.229 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

39 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
38 more

26 mentions without citation

Use of the spliceosomal protein U1A to facilitate crystallization and structure determination of complex RNAs.
Ferré-D'Amaré AR. (2010)
25 more