› 1m73

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF HUMAN PNP AT 2.3A RESOLUTION

Released:
DOI: 10.2210/pdb1m73/pdb
PDB entry 1m73 coloured by chain, front view.
PDB entry 1m73 coloured by chain, side view.
PDB entry 1m73 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human purine nucleoside phosphorylase at 2.3A resolution.
de Azevedo WF, Canduri F, dos Santos DM, Silva RG, de Oliveira JS, de Carvalho LP, Basso LA, Mendes MA, Palma MS, Santos DS
Biochem Biophys Res Commun 308 545-52 (2003)
PMID: 12914785

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: E
Molecule details ›
Chain: E
Length: 288 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 2-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: R32
Unit cell:
a: 141.63Å b: 141.63Å c: 163.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.205 0.222
Expression system: Escherichia coli

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Citations

24 citation in other articles

Altered enthalpy-entropy compensation in picomolar transition state analogues of human purine nucleoside phosphorylase.
Edwards et al. (2009)
23 more

6 mentions without citation

Four generations of transition-state analogues for human purine nucleoside phosphorylase.
Ho et al. (2010)
5 more