› 1ma9

X-ray diffraction
2.4Å resolution

Crystal structure of the complex of human vitamin D binding protein and rabbit muscle actin

Released:
DOI: 10.2210/pdb1ma9/pdb
PDB entry 1ma9 coloured by chain, front view.
PDB entry 1ma9 coloured by chain, side view.
PDB entry 1ma9 coloured by chain, top view.
Source organisms:
Primary publication:
Actin-DBP: the perfect structural fit?
Verboven C, Bogaerts I, Waelkens E, Rabijns A, Van Baelen H, Bouillon R, De Ranter C
Acta Crystallogr D Biol Crystallogr 59 263-73 (2003)
PMID: 12554937

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136196 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vitamin D-binding protein Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.29 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P02774 (Residues: 17-474; Coverage: 100%)
Gene name: GC
Sequence domains:
Structure domains: Serum Albumin; Chain A, Domain 1
Actin, alpha skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 74.44Å b: 74.9Å c: 88.02Å
α: 90° β: 110.19° γ: 90°
R-values:
R R work R free
0.203 0.2 0.25

Quick links

Citations

7 review citations

Therapeutic potential of vitamin D-binding protein.
Gomme et al. (2004)
6 more

9 mentions without citation

Molecular basis for G-actin binding to RPEL motifs from the serum response factor coactivator MAL.
Mouilleron et al. (2008)
8 more