› 1md8

X-ray diffraction
2.8Å resolution

Monomeric structure of the active catalytic domain of complement protease C1r

Released:
DOI: 10.2210/pdb1md8/pdb
PDB entry 1md8 coloured by chain, front view.
PDB entry 1md8 coloured by chain, side view.
PDB entry 1md8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Monomeric structures of the zymogen and active catalytic domain of complement protease c1r: further insights into the c1 activation mechanism.
Budayova-Spano M, Grabarse W, Thielens NM, Hillen H, Lacroix M, Schmidt M, Fontecilla-Camps JC, Arlaud GJ, Gaboriaud C
Structure 10 1509-19 (2002)
PMID: 12429092

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Lys(or Arg)-|-Ile bond in complement subcomponent C1s to form C1s (EC 3.4.21.42).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133182 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement C1r subcomponent heavy chain Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 37.2 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P00736 (Residues: 375-703; Coverage: 48%)
Gene name: C1R
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 112.911Å b: 49.459Å c: 77.249Å
α: 90° β: 106.19° γ: 90°
R-values:
R R work R free
0.204 0.198 0.269
Expression system: Trichoplusia ni

Quick links

Citations

13 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
12 more

12 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
11 more