› 1miu

X-ray diffraction
3.1Å resolution

Structure of a BRCA2-DSS1 complex

Released:
DOI: 10.2210/pdb1miu/pdb
PDB entry 1miu coloured by chain, front view.
PDB entry 1miu coloured by chain, side view.
PDB entry 1miu coloured by chain, top view.
Source organisms:
Primary publication:
BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure.
Yang H, Jeffrey PD, Miller J, Kinnucan E, Sun Y, Thoma NH, Zheng N, Chen PL, Lee WH, Pavletich NP
Science 297 1837-48 (2002)
PMID: 12228710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-158122 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
26S proteasome complex subunit SEM1 Chain: B
Molecule details ›
Chain: B
Length: 70 amino acids
Theoretical weight: 8.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P60896 (Residues: 1-70; Coverage: 100%)
Gene names: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Sequence domains: DSS1/SEM1 family
Breast cancer type 2 susceptibility protein homolog Chain: A
Molecule details ›
Chain: A
Length: 738 amino acids
Theoretical weight: 83.35 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P97929 (Residues: 2378-3115; Coverage: 22%)
Gene names: Brca2, Fancd1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand HG 5 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C222
Unit cell:
a: 160.509Å b: 228.271Å c: 81.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.256 0.31
Expression system: Spodoptera frugiperda

Quick links

Citations

132 review citations

Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints.
Sancar et al. (2004)
131 more

21 mentions without citation

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
20 more