› 1mjv

X-ray diffraction
2.1Å resolution

DISULFIDE DEFICIENT MUTANT OF VASCULAR ENDOTHELIAL GROWTH FACTOR A (C51A and C60A)

Released:
DOI: 10.2210/pdb1mjv/pdb
PDB entry 1mjv coloured by chain, front view.
PDB entry 1mjv coloured by chain, side view.
PDB entry 1mjv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The cystine knot promotes folding and not thermodynamic stability in vascular endothelial growth factor.
Muller YA, Heiring C, Misselwitz R, Welfle K, Welfle H
J Biol Chem 277 43410-6 (2002)
PMID: 12207021

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-VEGF Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15692 (Residues: 220-314; Coverage: 24%)
Gene names: VEGF, VEGFA
Structure domains: Cystine-knot cytokines

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 29.28Å b: 77.2Å c: 55.11Å
α: 90° β: 98.92° γ: 90°
R-values:
R R work R free
0.203 0.196 0.248
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Challenges and opportunities for non-antibody scaffold drugs.
Vazquez-Lombardi et al. (2015)
3 more

3 mentions without citation

Mechanical strength of 17,134 model proteins and cysteine slipknots.
Sikora et al. (2009)
2 more