› 1mkj

X-ray diffraction
2.7Å resolution

Human Kinesin Motor Domain With Docked Neck Linker

Released:
DOI: 10.2210/pdb1mkj/pdb
PDB entry 1mkj coloured by chain, front view.
PDB entry 1mkj coloured by chain, side view.
PDB entry 1mkj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy.
Sindelar CV, Budny MJ, Rice S, Naber N, Fletterick R, Cooke R
Nat Struct Biol 9 844-8 (2002)
PMID: 12368902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-1 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P33176 (Residues: 1-349; Coverage: 36%)
Gene names: KIF5B, KNS, KNS1
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand SO4 2 x SO4
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 73.796Å b: 74.086Å c: 91.543Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The role of microtubules in processive kinesin movement.
Kikkawa M. (2008)
3 more

31 mentions without citation

Kinesin, 30 years later: Recent insights from structural studies.
Wang et al. (2015)
30 more