› 1mlc

X-ray diffraction
2.5Å resolution

MONOCLONAL ANTIBODY FAB D44.1 RAISED AGAINST CHICKEN EGG-WHITE LYSOZYME COMPLEXED WITH LYSOZYME

Released:
DOI: 10.2210/pdb1mlc/pdb
PDB entry 1mlc coloured by chain, front view.
PDB entry 1mlc coloured by chain, side view.
PDB entry 1mlc coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structures of the free and the antigen-complexed Fab from monoclonal anti-lysozyme antibody D44.1.
Braden BC, Souchon H, Eiselé JL, Bentley GA, Bhat TN, Navaza J, Poljak RJ
J Mol Biol 243 767-81 (1994)
PMID: 7966295

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132845 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.61 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-1 chain C region secreted form Chains: B, D
Molecule details ›
Chains: B, D
Length: 218 amino acids
Theoretical weight: 23.22 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P01868 (Residues: 1-102; Coverage: 32%)
Gene names: Igh-4, Ighg1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Lysozyme C Chains: E, F
Molecule details ›
Chains: E, F
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 99.7Å b: 167.3Å c: 84.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 not available 0.282
Expression system: Not provided

Quick links

Citations

9 review citations

Interactions of protein antigens with antibodies.
Davies et al. (1996)
8 more

66 mentions without citation

Single domain antibodies: promising experimental and therapeutic tools in infection and immunity.
Wesolowski et al. (2009)
65 more