› 1mlw

X-ray diffraction
1.71Å resolution

Crystal structure of human tryptophan hydroxylase with bound 7,8-dihydro-L-biopterin cofactor and Fe(III)

Released:
DOI: 10.2210/pdb1mlw/pdb
PDB entry 1mlw coloured by chain, front view.
PDB entry 1mlw coloured by chain, side view.
PDB entry 1mlw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional structure of human tryptophan hydroxylase and its implications for the biosynthesis of the neurotransmitters serotonin and melatonin.
Wang L, Erlandsen H, Haavik J, Knappskog PM, Stevens RC
Biochemistry 41 12569-74 (2002)
PMID: 12379098

Function and Biology Details

Reaction catalysed: 
L-tryptophan + tetrahydrobiopterin + O(2) = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148158 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 5-hydroxylase 1 Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P17752 (Residues: 102-402; Coverage: 68%)
Gene names: TPH, TPH1, TPRH, TRPH
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments


Cofactor: Ligand HBI 1 x HBI
1 bound ligand:
Ligand FE 1 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 47.775Å b: 57.334Å c: 109.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.23
Expression system: Escherichia coli

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Citations

10 review citations

Mononuclear non-heme iron enzymes with the 2-His-1-carboxylate facial triad: recent developments in enzymology and modeling studies.
Bruijnincx et al. (2008)
9 more

13 mentions without citation

Mechanism of aromatic amino acid hydroxylation.
Fitzpatrick PF. (2003)
12 more