› 1mm2

Solution NMR

Solution structure of the 2nd PHD domain from Mi2b

Released:
DOI: 10.2210/pdb1mm2/pdb
PDB entry 1mm2 coloured by chain, ensemble of 20 models, front view.
PDB entry 1mm2 coloured by chain, ensemble of 20 models, side view.
PDB entry 1mm2 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Engineering a protein scaffold from a PHD finger.
Kwan AH, Gell DA, Verger A, Crossley M, Matthews JM, Mackay JP
Structure 11 803-13 (2003)
PMID: 12842043

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromodomain-helicase-DNA-binding protein 4 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14839 (Residues: 446-501; Coverage: 3%)
Gene name: CHD4
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Engineering novel binding proteins from nonimmunoglobulin domains.
Binz et al. (2005)
15 more

8 mentions without citation

Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9.
Mansfield et al. (2011)
7 more