› 1mmb

X-ray diffraction
2.1Å resolution

COMPLEX OF BB94 WITH THE CATALYTIC DOMAIN OF MATRIX METALLOPROTEINASE-8

Released:
DOI: 10.2210/pdb1mmb/pdb
PDB entry 1mmb coloured by chain, front view.
PDB entry 1mmb coloured by chain, side view.
PDB entry 1mmb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure determination and analysis of human neutrophil collagenase complexed with a hydroxamate inhibitor.
Grams F, Crimmin M, Hinnes L, Huxley P, Pieper M, Tschesche H, Bode W
Biochemistry 34 14012-20 (1995)
PMID: 7577999

Function and Biology Details

Reaction catalysed: 
Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil collagenase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22894 (Residues: 100-262; Coverage: 37%)
Gene names: CLG1, MMP8
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
Ligand BAT 1 x BAT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 33.67Å b: 69.64Å c: 73.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 not available
Expression system: Escherichia coli

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Citations

13 review citations

Critical appraisal of the use of matrix metalloproteinase inhibitors in cancer treatment.
Zucker et al. (2000)
12 more

7 mentions without citation

Database of traditional Chinese medicine and its application to studies of mechanism and to prescription validation.
Chen et al. (2006)
6 more