› 1mnc

X-ray diffraction
2.1Å resolution

STRUCTURE OF HUMAN NEUTROPHIL COLLAGENASE REVEALS LARGE S1' SPECIFICITY POCKET

Released:
DOI: 10.2210/pdb1mnc/pdb
PDB entry 1mnc coloured by chain, front view.
PDB entry 1mnc coloured by chain, side view.
PDB entry 1mnc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human neutrophil collagenase reveals large S1' specificity pocket.
Stams T, Spurlino JC, Smith DL, Wahl RC, Ho TF, Qoronfleh MW, Banks TM, Rubin B
Nat Struct Biol 1 119-23 (1994)
PMID: 7656015

Function and Biology Details

Reaction catalysed: 
Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil collagenase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 17.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P22894 (Residues: 101-263; Coverage: 37%)
Gene names: CLG1, MMP8
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 1 x CA
Ligand PLH 1 x PLH
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 34.92Å b: 61.28Å c: 68.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 not available
Expression system: Not provided

Quick links

Citations

19 review citations

Matrix metalloproteinases: structures, evolution, and diversification.
Massova et al. (1998)
18 more

9 mentions without citation

Meprins, membrane-bound and secreted astacin metalloproteinases.
Sterchi et al. (2008)
8 more