› 1mp0

X-ray diffraction
2.2Å resolution

Binary Complex of Human Glutathione-Dependent Formaldehyde Dehydrogenase with NAD(H)

Released:
DOI: 10.2210/pdb1mp0/pdb
PDB entry 1mp0 coloured by chain, front view.
PDB entry 1mp0 coloured by chain, side view.
PDB entry 1mp0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function relationships in human Class III alcohol dehydrogenase (formaldehyde dehydrogenase).
Sanghani PC, Robinson H, Bennett-Lovsey R, Hurley TD, Bosron WF
Chem Biol Interact 143-144 195-200 (2003)
PMID: 12604204

Function and Biology Details

Reactions catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase class-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 39.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11766 (Residues: 2-374; Coverage: 100%)
Gene names: ADH5, ADHX, FDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand ZN 4 x ZN
Ligand K 2 x K
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P43212
Unit cell:
a: 79.033Å b: 79.033Å c: 311.755Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Merging protein, gene and genomic data: the evolution of the MDR-ADH family.
Gonzàlez-Duarte et al. (2005)
2 more

4 mentions without citation

S-nitrosoglutathione reductases are low-copy number, cysteine-rich proteins in plants that control multiple developmental and defense responses in Arabidopsis.
Xu et al. (2013)
3 more