› 1mr3

X-ray diffraction
1.6Å resolution

Saccharomyces cerevisiae ADP-ribosylation Factor 2 (ScArf2) complexed with GDP-3'P at 1.6A resolution

Released:
DOI: 10.2210/pdb1mr3/pdb
PDB entry 1mr3 coloured by chain, front view.
PDB entry 1mr3 coloured by chain, side view.
PDB entry 1mr3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of yeast ARF2 and ARL1: distinct roles for the N terminus in the structure and function of ARF family GTPases.
Amor JC, Horton JR, Zhu X, Wang Y, Sullards C, Ringe D, Cheng X, Kahn RA
J Biol Chem 276 42477-84 (2001)
PMID: 11535602

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 2 Chain: F
Molecule details ›
Chain: F
Length: 181 amino acids
Theoretical weight: 20.68 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19146 (Residues: 1-181; Coverage: 100%)
Gene names: ARF2, D2165, YDL137W
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

6 bound ligands:
Ligand MG 1 x MG
Ligand G3D 1 x G3D
Ligand EDO 10 x EDO
Ligand GOL 2 x GOL
Ligand EOH 4 x EOH
Ligand PDO 2 x PDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21212
Unit cell:
a: 61.822Å b: 69.47Å c: 40.861Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Arf and its many interactors.
Nie et al. (2003)
4 more

4 mentions without citation

Dynamic structure of membrane-anchored Arf*GTP.
Liu et al. (2010)
3 more