› 1mt6

X-ray diffraction
2.2Å resolution

Structure of histone H3 K4-specific methyltransferase SET7/9 with AdoHcy

Released:
DOI: 10.2210/pdb1mt6/pdb
PDB entry 1mt6 coloured by chain, front view.
PDB entry 1mt6 coloured by chain, side view.
PDB entry 1mt6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The active site of the SET domain is constructed on a knot.
Jacobs SA, Harp JM, Devarakonda S, Kim Y, Rastinejad F, Khorasanizadeh S
Nat Struct Biol 9 833-8 (2002)
PMID: 12389038

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase SETD7 Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WTS6 (Residues: 58-337; Coverage: 77%)
Gene names: KIAA1717, KMT7, SET7, SET9, SETD7
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I222
Unit cell:
a: 70.347Å b: 76.038Å c: 166.284Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The SET-domain protein superfamily: protein lysine methyltransferases.
Dillon et al. (2005)
13 more

12 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
11 more