› 1muj

X-ray diffraction
2.15Å resolution

Crystal structure of murine class II MHC I-Ab in complex with a human CLIP peptide

Released:
DOI: 10.2210/pdb1muj/pdb
PDB entry 1muj coloured by chain, front view.
PDB entry 1muj coloured by chain, side view.
PDB entry 1muj coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif.
Zhu Y, Rudensky AY, Corper AL, Teyton L, Wilson IA
J Mol Biol 326 1157-74 (2003)
PMID: 12589760

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137530 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class II histocompatibility antigen, A-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.43 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P14434 (Residues: 24-205; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
H-2 class II histocompatibility antigen, A beta chain Chain: B
Molecule details ›
Chain: B
Length: 197 amino acids
Theoretical weight: 23.19 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P14483 (Residues: 28-216; Coverage: 79%)
Gene names: H2-Ab1, H2-iabeta
Sequence domains:
Structure domains:
Class-II-associated invariant chain peptide Chain: C
Molecule details ›
Chain: C
Length: 36 amino acids
Theoretical weight: 3.7 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P04233 (Residues: 97-122; Coverage: 9%)
Gene names: CD74, DHLAG

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 63.647Å b: 88.065Å c: 83.74Å
α: 90° β: 92.48° γ: 90°
R-values:
R R work R free
0.22 0.22 0.246
Expression system: Drosophila melanogaster

Quick links

Citations

10 review citations

Cell biology of antigen processing in vitro and in vivo.
Trombetta et al. (2005)
9 more

11 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
10 more