› 1n0x

X-ray diffraction
1.8Å resolution

Crystal Structure of a Broadly Neutralizing Anti-HIV-1 Antibody in Complex with a Peptide Mimotope

Released:
DOI: 10.2210/pdb1n0x/pdb
PDB entry 1n0x coloured by chain, front view.
PDB entry 1n0x coloured by chain, side view.
PDB entry 1n0x coloured by chain, top view.
Source organisms:
Entry authors: Saphire EO, Montero M, Menendez A, Irving MB, Zwick MB, Parren PWHI, Burton DR, Scott JK, Wilson IA

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-134648 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chains: L, M
Molecule details ›
Chains: L, M
Length: 215 amino acids
Theoretical weight: 23.71 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P01834 (Residues: 1-107; Coverage: 100%)
Gene name: IGKC
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin gamma-1 heavy chain Chains: H, K
Molecule details ›
Chains: H, K
Length: 230 amino acids
Theoretical weight: 24.94 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 1-49, 50-99, 100-104, 105-222; Coverage: 49%)
Sequence domains:
Structure domains: Immunoglobulins
B2.1 peptide Chains: P, R
Molecule details ›
Chains: P, R
Length: 21 amino acids
Theoretical weight: 2.55 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 4 x SO4
Ligand CXS 1 x CXS
Ligand K 1 x K
1 modified residue:
Ligand ORN 2 x ORN

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 51.621Å b: 184.358Å c: 56.173Å
α: 90° β: 103.05° γ: 90°
R-values:
R R work R free
0.229 0.22 0.252
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links