› 1n19

X-ray diffraction
1.86Å resolution

Structure of the HSOD A4V mutant

Released:
DOI: 10.2210/pdb1n19/pdb
PDB entry 1n19 coloured by chain, front view.
PDB entry 1n19 coloured by chain, side view.
PDB entry 1n19 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into Lou Gehrig's disease from the structure and instability of the A4V mutant of human Cu,Zn superoxide dismutase.
Cardoso RM, Thayer MM, DiDonato M, Lo TP, Bruns CK, Getzoff ED, Tainer JA
J Mol Biol 324 247-56 (2002)
PMID: 12441104

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 15.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00441 (Residues: 1-154; Coverage: 100%)
Gene name: SOD1
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

3 bound ligands:
Ligand CU1 2 x CU1
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 114.7Å b: 47.9Å c: 56.2Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.207 0.207 0.26
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Copper-zinc superoxide dismutase and amyotrophic lateral sclerosis.
Valentine et al. (2005)
9 more

6 mentions without citation

Structural characterization of zinc-deficient human superoxide dismutase and implications for ALS.
Roberts et al. (2007)
5 more