› 1n6a

X-ray diffraction
1.7Å resolution

Structure of SET7/9

Released:
DOI: 10.2210/pdb1n6a/pdb
PDB entry 1n6a coloured by chain, front view.
PDB entry 1n6a coloured by chain, side view.
PDB entry 1n6a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism of histone lysine methyl transfer revealed by the structure of SET7/9-AdoMet.
Kwon T, Chang JH, Kwak E, Lee CW, Joachimiak A, Kim YC, Lee J, Cho Y
EMBO J 22 292-303 (2003)
PMID: 12514135

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186700 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase SETD7 Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 29.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WTS6 (Residues: 108-366; Coverage: 71%)
Gene names: KIAA1717, KMT7, SET7, SET9, SETD7
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAM 1 x SAM
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 102.231Å b: 38.668Å c: 66.596Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.194 0.217
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
17 more

9 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
8 more