› 1n6j

X-ray diffraction
2.2Å resolution

Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2

Released:
DOI: 10.2210/pdb1n6j/pdb
PDB entry 1n6j coloured by chain, ensemble of 2 models, front view.
PDB entry 1n6j coloured by chain, ensemble of 2 models, side view.
PDB entry 1n6j coloured by chain, ensemble of 2 models, top view.
Source organism: Homo sapiens
Primary publication:
Sequence-specific recruitment of transcriptional co-repressor Cabin1 by myocyte enhancer factor-2.
Han A, Pan F, Stroud JC, Youn HD, Liu JO, Chen L
Nature 422 730-4 (2003)
PMID: 12700764

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114235 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Myocyte-specific enhancer factor 2B Chains: A, B
Molecule details ›
Chains: A, B
Length: 93 amino acids
Theoretical weight: 10.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02080 (Residues: 2-94; Coverage: 26%)
Gene names: MEF2B, XMEF2
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
Calcineurin-binding protein cabin-1 Chain: G
Molecule details ›
Chain: G
Length: 35 amino acids
Theoretical weight: 3.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6J0 (Residues: 2156-2190; Coverage: 2%)
Gene names: CABIN1, KIAA0330
Sequence domains: MEF2 binding
5'-D(*AP*GP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
5'-D(*GP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.28 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P4122
Unit cell:
a: 70.14Å b: 70.14Å c: 151.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.268
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Control of cardiac growth by histone acetylation/deacetylation.
Backs et al. (2006)
16 more

15 mentions without citation

Structural Basis for Plant MADS Transcription Factor Oligomerization.
Lai et al. (2019)
14 more