› 1n7m

X-ray diffraction
1.8Å resolution

Germline 7G12 with N-methylmesoporphyrin

Released:
DOI: 10.2210/pdb1n7m/pdb
PDB entry 1n7m coloured by chain, front view.
PDB entry 1n7m coloured by chain, side view.
PDB entry 1n7m coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural evidence for substrate strain in antibody catalysis.
Yin J, Andryski SE, Beuscher AE, Stevens RC, Schultz PG
Proc Natl Acad Sci U S A 100 856-61 (2003)
PMID: 12552112

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208623 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Germline Metal Chelatase Catalytic Antibody, chain H Chain: H
Molecule details ›
Chain: H
Length: 213 amino acids
Theoretical weight: 23.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01857 (Residues: 1-102; Coverage: 26%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand MMP 1 x MMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 55.274Å b: 76.236Å c: 60.435Å
α: 90° β: 93.33° γ: 90°
R-values:
R R work R free
0.22 0.22 0.253
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mechanical processes in biochemistry.
Bustamante et al. (2004)
2 more

6 mentions without citation

Diverse specificity and effector function among human antibodies to HIV-1 envelope glycoprotein epitopes exposed by CD4 binding.
Guan et al. (2013)
5 more