› 1nbz

X-ray diffraction
1.85Å resolution

Crystal Structure of HyHEL-63 complexed with HEL mutant K97A

Released:
DOI: 10.2210/pdb1nbz/pdb
PDB entry 1nbz coloured by chain, front view.
PDB entry 1nbz coloured by chain, side view.
PDB entry 1nbz coloured by chain, top view.
Source organisms:
Primary publication:
Dissection of binding interactions in the complex between the anti-lysozyme antibody HyHEL-63 and its antigen.
Li Y, Urrutia M, Smith-Gill SJ, Mariuzza RA
Biochemistry 42 11-22 (2003)
PMID: 12515535

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-132846 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 210 amino acids
Theoretical weight: 22.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q569W9 (Residues: 20-70, 71-235; Coverage: 47%)
Gene name: Igh
Sequence domains:
Structure domains: Immunoglobulins
Lysozyme C Chain: C
Molecule details ›
Chain: C
Length: 129 amino acids
Theoretical weight: 14.27 KDa
Source organism: Gallus gallus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42212
Unit cell:
a: 90.903Å b: 90.903Å c: 151.184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.229
Expression systems:
  • Escherichia coli
  • Saccharomyces cerevisiae

Quick links

Citations

7 review citations

Pediocin-like antimicrobial peptides (class IIa bacteriocins) and their immunity proteins: biosynthesis, structure, and mode of action.
Fimland et al. (2005)
6 more

1 mention without citation

A meta-learning approach for B-cell conformational epitope prediction.
Hu et al. (2014)