› 1nmd

X-ray diffraction
1.9Å resolution

Crystal Structure of D. Discoideum Actin-Gelsolin Segment 1 Complex Crystallized In Presence Of Lithium ATP

Released:
DOI: 10.2210/pdb1nmd/pdb
PDB entry 1nmd coloured by chain, front view.
PDB entry 1nmd coloured by chain, side view.
PDB entry 1nmd coloured by chain, top view.
Source organisms:
Primary publication:
The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.
Vorobiev S, Strokopytov B, Drubin DG, Frieden C, Ono S, Condeelis J, Rubenstein PA, Almo SC
Proc Natl Acad Sci U S A 100 5760-5 (2003)
PMID: 12732734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Major actin Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.65 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P07830 (Residues: 2-376; Coverage: 100%)
Gene names: DDB_G0269234, DDB_G0272248, DDB_G0272520, DDB_G0274129, DDB_G0274133, DDB_G0274135, DDB_G0274137, DDB_G0274285, DDB_G0274561, DDB_G0274599, DDB_G0274601, DDB_G0274727, DDB_G0280545, DDB_G0288879, DDB_G0289005, DDB_G0289553, DDB_G0289663, act1, act11, act12, act13, act14, act15, act16, act19, act1a, act2, act2-1, act20, act21, act4, act5, act6, act7, act8, act9, actA1, actA8, actB1, actM6
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 125 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand ATP 1 x ATP
Ligand SO2 1 x SO2
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 178.912Å b: 69.053Å c: 56.613Å
α: 90° β: 104.65° γ: 90°
R-values:
R R work R free
0.203 0.203 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
8 more

5 mentions without citation

The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.
Vorobiev et al. (2003)
4 more