› 1nsk

X-ray diffraction
2.8Å resolution

THE CRYSTAL STRUCTURE OF A HUMAN NUCLEOSIDE DIPHOSPHATE KINASE, NM23-H2

Released:
DOI: 10.2210/pdb1nsk/pdb
PDB entry 1nsk coloured by chain, front view.
PDB entry 1nsk coloured by chain, side view.
PDB entry 1nsk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of a human nucleoside diphosphate kinase, NM23-H2.
Webb PA, Perisic O, Mendola CE, Backer JM, Williams RL
J Mol Biol 251 574-87 (1995)
PMID: 7658474

Function and Biology Details

Reactions catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase B Chains: L, N, O, R, T, U
Molecule details ›
Chains: L, N, O, R, T, U
Length: 152 amino acids
Theoretical weight: 17.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22392 (Residues: 1-152; Coverage: 100%)
Gene names: NM23B, NME2
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 118.67Å b: 118.8Å c: 90.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.294
Expression system: Escherichia coli

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Citations

18 review citations

The human Nm23/nucleoside diphosphate kinases.
Lacombe et al. (2000)
17 more

6 mentions without citation

Protein Ensembles: How Does Nature Harness Thermodynamic Fluctuations for Life? The Diverse Functional Roles of Conformational Ensembles in the Cell.
Wei et al. (2016)
5 more