› 1nub

X-ray diffraction
2.8Å resolution

HELIX C DELETION MUTANT OF BM-40 FS-EC DOMAIN PAIR

Released:
DOI: 10.2210/pdb1nub/pdb
PDB entry 1nub coloured by chain, front view.
PDB entry 1nub coloured by chain, side view.
PDB entry 1nub coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure and mapping by site-directed mutagenesis of the collagen-binding epitope of an activated form of BM-40/SPARC/osteonectin.
Sasaki T, Hohenester E, Göhring W, Timpl R
EMBO J 17 1625-34 (1998)
PMID: 9501084

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
SPARC Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P09486 (Residues: 71-303; Coverage: 79%)
Gene names: ON, SPARC
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-21
Spacegroup: P212121
Unit cell:
a: 51.86Å b: 88.14Å c: 153.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.306
Expression system: Homo sapiens

Quick links

Citations

20 review citations

SPARC, a matricellular protein: at the crossroads of cell-matrix communication.
Brekken et al. (2001)
19 more

4 mentions without citation

Expression, functional, and structural analysis of proteins critical for otoconia development.
Xu et al. (2010)
3 more