› 1nup

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF HUMAN CYTOSOLIC NMN/NaMN ADENYLYLTRANSFERASE COMPLEX WITH NMN

Released:
DOI: 10.2210/pdb1nup/pdb
PDB entry 1nup coloured by chain, front view.
PDB entry 1nup coloured by chain, side view.
PDB entry 1nup coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis.
Zhang X, Kurnasov OV, Karthikeyan S, Grishin NV, Osterman AL, Zhang H
J Biol Chem 278 13503-11 (2003)
PMID: 12574164

Function and Biology Details

Reactions catalysed: 
ATP + nicotinamide ribonucleotide = diphosphate + NAD(+)
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188765 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96T66 (Residues: 1-252; Coverage: 100%)
Gene names: FKSG76, NMNAT3
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NMN 2 x NMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 79.541Å b: 79.541Å c: 146.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.2 0.242
Expression system: Escherichia coli

Quick links

Citations

29 review citations

NAD(+) Metabolism and the Control of Energy Homeostasis: A Balancing Act between Mitochondria and the Nucleus.
Cantó et al. (2015)
28 more

1 mention without citation

Homology modeling and deletion mutants of human nicotinamide mononucleotide adenylyltransferase isozyme 2: new insights on structure and function relationship.
Brunetti et al. (2010)