› 1nw1

X-ray diffraction
2.02Å resolution

Crystal Structure of Choline Kinase

Released:
DOI: 10.2210/pdb1nw1/pdb
PDB entry 1nw1 coloured by chain, front view.
PDB entry 1nw1 coloured by chain, side view.
PDB entry 1nw1 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
The crystal structure of choline kinase reveals a eukaryotic protein kinase fold.
Peisach D, Gee P, Kent C, Xu Z
Structure 11 703-13 (2003)
PMID: 12791258

Function and Biology Details

Reactions catalysed: 
ATP + choline = ADP + phosphocholine
ATP + ethanolamine = ADP + O-phosphoethanolamine
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline kinase A2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 49.3 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q22942 (Residues: 1-429; Coverage: 100%)
Gene names: C52B9.1, cka-2
Sequence domains: Choline/ethanolamine kinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 55.372Å b: 102.141Å c: 85.369Å
α: 90° β: 90.66° γ: 90°
R-values:
R R work R free
0.209 0.209 0.231
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Phospholipid biosynthesis in mammalian cells.
Vance et al. (2004)
10 more

9 mentions without citation

Structural evolution of the protein kinase-like superfamily.
Scheeff et al. (2005)
8 more