› 1o4x

Solution NMR

TERNARY COMPLEX OF THE DNA BINDING DOMAINS OF THE OCT1 AND SOX2 TRANSCRIPTION FACTORS WITH A 19MER OLIGONUCLEOTIDE FROM THE HOXB1 REGULATORY ELEMENT

Released:
DOI: 10.2210/pdb1o4x/pdb
PDB entry 1o4x coloured by chain, front view.
PDB entry 1o4x coloured by chain, side view.
PDB entry 1o4x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for synergistic transcriptional activation by Oct1 and Sox2 revealed from the solution structure of the 42-kDa Oct1.Sox2.Hoxb1-DNA ternary transcription factor complex.
Williams DC, Cai M, Clore GM
J Biol Chem 279 1449-57 (2004)
PMID: 14559893

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114296 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
POU domain, class 2, transcription factor 1 Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14859 (Residues: 280-442; Coverage: 22%)
Gene names: OCT1, OTF1, POU2F1
Sequence domains:
Structure domains:
Transcription factor SOX-2 Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48431 (Residues: 39-121; Coverage: 26%)
Gene name: SOX2
Sequence domains: HMG (high mobility group) box
Structure domains: High mobility group box domain
5'-D(*TP*GP*TP*CP*TP*TP*TP*GP*TP*CP*AP*TP*GP*CP*TP*AP*AP*TP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.82 KDa
5'-D(*CP*AP*TP*TP*AP*GP*CP*AP*TP*GP*AP*CP*AP*AP*AP*GP*AP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.83 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: CONJOINED RIGID BODY/TORSION ANGLE DYNAMICS
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
20 more

21 mentions without citation

SOX2 protein biochemistry in stemness, reprogramming, and cancer: the PI3K/AKT/SOX2 axis and beyond.
Schaefer et al. (2020)
20 more