› 1oai

X-ray diffraction
1Å resolution

Complex between Tap UBA domain and FxFG nucleoporin peptide

Released:
DOI: 10.2210/pdb1oai/pdb
PDB entry 1oai coloured by chain, front view.
PDB entry 1oai coloured by chain, side view.
PDB entry 1oai coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the interaction between the Tap/NXF1 UBA domain and FG nucleoporins at 1A resolution.
Grant RP, Neuhaus D, Stewart M
J Mol Biol 326 849-58 (2003)
PMID: 12581645

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162349 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear RNA export factor 1 Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBU9 (Residues: 561-619; Coverage: 10%)
Gene names: NXF1, TAP
Sequence domains: TAP C-terminal domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
FXFG NUCLEOPORIN PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P3121
Unit cell:
a: 42.974Å b: 42.974Å c: 66.852Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.149 0.149 0.159
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Molecular mechanism of the nuclear protein import cycle.
Stewart M. (2007)
14 more

22 mentions without citation

Mechanisms of nuclear mRNA export: A structural perspective.
Xie et al. (2019)
21 more