› 1oes

X-ray diffraction
2.2Å resolution

Oxidation state of protein tyrosine phosphatase 1B

Released:
DOI: 10.2210/pdb1oes/pdb
PDB entry 1oes coloured by chain, front view.
PDB entry 1oes coloured by chain, side view.
PDB entry 1oes coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Oxidation state of the active-site cysteine in protein tyrosine phosphatase 1B.
van Montfort RL, Congreve M, Tisi D, Carr R, Jhoti H
Nature 423 773-7 (2003)
PMID: 12802339

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 37.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18031 (Residues: 1-321; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P3121
Unit cell:
a: 87.686Å b: 87.686Å c: 103.721Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

156 review citations

Reactive oxygen species: metabolism, oxidative stress, and signal transduction.
Apel et al. (2004)
155 more

8 mentions without citation

Covalent inhibition of protein tyrosine phosphatases.
Ruddraraju et al. (2017)
7 more