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Solution NMR

NMR Structure of a Complex Containing the TFIIF Subunit RAP74 and the RNAP II CTD Phosphatase FCP1

Released:
DOI: 10.2210/pdb1onv/pdb
PDB entry 1onv coloured by chain, ensemble of 20 models, front view.
PDB entry 1onv coloured by chain, ensemble of 20 models, side view.
PDB entry 1onv coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1.
Nguyen BD, Abbott KL, Potempa K, Kobor MS, Archambault J, Greenblatt J, Legault P, Omichinski JG
Proc Natl Acad Sci U S A 100 5688-93 (2003)
PMID: 12732728

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152878 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
General transcription factor IIF subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35269 (Residues: 436-517; Coverage: 16%)
Gene names: GTF2F1, RAP74
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
RNA polymerase II subunit A C-terminal domain phosphatase Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5B0 (Residues: 879-961; Coverage: 9%)
Gene names: CTDP1, FCP1
Sequence domains: FCP1, C-terminal

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Structure and mechanism of the RNA polymerase II transcription machinery.
Hahn S. (2004)
3 more

2 mentions without citation

NMR structure of a complex containing the TFIIF subunit RAP74 and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1.
Nguyen et al. (2003)
1 more