› 1opi

Solution NMR

SOLUTION STRUCTURE OF THE THIRD RNA RECOGNITION MOTIF (RRM) OF U2AF65 IN COMPLEX WITH AN N-TERMINAL SF1 PEPTIDE

Released:
DOI: 10.2210/pdb1opi/pdb
PDB entry 1opi coloured by chain, ensemble of 10 models, front view.
PDB entry 1opi coloured by chain, ensemble of 10 models, side view.
PDB entry 1opi coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP.
Selenko P, Gregorovic G, Sprangers R, Stier G, Rhani Z, Krämer A, Sattler M
Mol Cell 11 965-76 (2003)
PMID: 12718882

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150720 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 11.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26368 (Residues: 372-475; Coverage: 22%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Splicing factor 1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15637 (Residues: 13-25; Coverage: 2%)
Gene names: SF1, ZFM1, ZNF162

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RESTRAINED MOLECULAR DYNAMICS USING CNS AND ARIA FOR AMBIGUOUS DISTANCE RESTRAINTS
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

26 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
25 more

6 mentions without citation

Phosphorylation of S776 and 14-3-3 binding modulate ataxin-1 interaction with splicing factors.
de Chiara et al. (2009)
5 more