› 1orq

X-ray diffraction
3.2Å resolution

X-ray structure of a voltage-dependent potassium channel in complex with an Fab

Released:
DOI: 10.2210/pdb1orq/pdb
PDB entry 1orq coloured by chain, front view.
PDB entry 1orq coloured by chain, side view.
PDB entry 1orq coloured by chain, top view.
Source organisms:
Primary publication:
X-ray structure of a voltage-dependent K+ channel.
Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, MacKinnon R
Nature 423 33-41 (2003)
PMID: 12721618

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dodecamer (preferred)
hetero 24-mer
PDBe Complex ID:
PDB-CPX-134706 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa constant Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.53 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ig gamma-2A chain C region, membrane-bound form Chain: B
Molecule details ›
Chain: B
Length: 219 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01865 (Residues: 1-99; Coverage: 25%)
Gene name: Igh-1a
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Voltage-gated potassium channel Chain: C
Molecule details ›
Chain: C
Length: 223 amino acids
Theoretical weight: 24.27 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9YDF8 (Residues: 31-253; Coverage: 76%)
Gene name: APE_0955
Sequence domains: Ion transport protein
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand CD 7 x CD
Ligand K 6 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I422
Unit cell:
a: 189.439Å b: 189.439Å c: 150.479Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.253 0.298
Expression system: Escherichia coli

Quick links

Citations

273 review citations

hERG potassium channels and cardiac arrhythmia.
Sanguinetti et al. (2006)
272 more

65 mentions without citation

Normal mode analysis of biomolecular structures: functional mechanisms of membrane proteins.
Bahar et al. (2010)
64 more