› 1ot8

X-ray diffraction
2Å resolution

Structure of the Ankyrin Domain of the Drosophila Notch Receptor

Released:
DOI: 10.2210/pdb1ot8/pdb
PDB entry 1ot8 coloured by chain, front view.
PDB entry 1ot8 coloured by chain, side view.
PDB entry 1ot8 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Structure and stability of the ankyrin domain of the Drosophila Notch receptor.
Zweifel ME, Leahy DJ, Hughson FM, Barrick D
Protein Sci 12 2622-32 (2003)
PMID: 14573873

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurogenic locus Notch protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 239 amino acids
Theoretical weight: 25.7 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07207 (Residues: 1902-2139; Coverage: 9%)
Gene names: CG3936, N
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 73.629Å b: 73.629Å c: 341.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.198
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

The ankyrin repeat as molecular architecture for protein recognition.
Mosavi et al. (2004)
9 more

22 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
21 more