1otu

X-ray diffraction
3.3Å resolution

Structure of the Escherichia coli ClC Chloride channel E148Q mutant and Fab Complex

Released:
Source organisms:
Primary publication:
Gating the selectivity filter in ClC chloride channels.
Science 300 108-12 (2003)
PMID: 12649487

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-134603 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 465 amino acids
Theoretical weight: 49.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-465; Coverage: 98%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel
Ig heavy chain V region T601 Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01808 (Residues: 1-101, 102-104, 105-118; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chains: D, F
Molecule details ›
Chains: D, F
Length: 211 amino acids
Theoretical weight: 23.09 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-104; Coverage: 97%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 232.113Å b: 96.09Å c: 170.284Å
α: 90° β: 131.39° γ: 90°
R-values:
R R work R free
0.294 0.294 0.325
Expression system: Escherichia coli BL21(DE3)