› 1p49

X-ray diffraction
2.6Å resolution

Structure of Human Placental Estrone/DHEA Sulfatase

Released:
DOI: 10.2210/pdb1p49/pdb
PDB entry 1p49 coloured by chain, front view.
PDB entry 1p49 coloured by chain, side view.
PDB entry 1p49 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human estrone sulfatase suggests functional roles of membrane association.
Hernandez-Guzman FG, Higashiyama T, Pangborn W, Osawa Y, Ghosh D
J Biol Chem 278 22989-97 (2003)
PMID: 12657638

Function and Biology Details

Reaction catalysed: 
3-beta-hydroxyandrost-5-en-17-one 3-sulfate + H(2)O = 3-beta-hydroxyandrost-5-en-17-one + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-140397 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steryl-sulfatase Chain: A
Molecule details ›
Chain: A
Length: 562 amino acids
Theoretical weight: 63.05 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P08842 (Residues: 22-583; Coverage: 100%)
Gene names: ARSC1, STS
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand BOG 2 x BOG
Ligand NAG 2 x NAG
Ligand CA 1 x CA
Ligand PO4 1 x PO4
1 modified residue:
Ligand ALS 1 x ALS

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P321
Unit cell:
a: 116.98Å b: 116.98Å c: 102.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.254 0.301

Quick links

Citations

28 review citations

Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility.
Hanson et al. (2004)
27 more

50 mentions without citation

Influences of membrane mimetic environments on membrane protein structures.
Zhou et al. (2013)
49 more