› 1p4m

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF RIBOFLAVIN KINASE

Released:
DOI: 10.2210/pdb1p4m/pdb
PDB entry 1p4m coloured by chain, front view.
PDB entry 1p4m coloured by chain, side view.
PDB entry 1p4m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch.
Karthikeyan S, Zhou Q, Mseeh F, Grishin NV, Osterman AL, Zhang H
Structure 11 265-73 (2003)
PMID: 12623014

Function and Biology Details

Reaction catalysed: 
ATP + riboflavin = ADP + FMN
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q969G6 (Residues: 2-148; Coverage: 95%)
Gene name: RFK
Sequence domains: Riboflavin kinase
Structure domains: Riboflavin kinase-like

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P3121
Unit cell:
a: 57.097Å b: 57.097Å c: 82.482Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.227
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Principles for targeting RNA with drug-like small molecules.
Warner et al. (2018)
7 more

7 mentions without citation

Development of Novel Experimental Models to Study Flavoproteome Alterations in Human Neuromuscular Diseases: The Effect of Rf Therapy.
Tolomeo et al. (2020)
6 more