› 1p4u

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF GGA3 GAE DOMAIN IN COMPLEX WITH RABAPTIN-5 PEPTIDE

Released:
DOI: 10.2210/pdb1p4u/pdb
PDB entry 1p4u coloured by chain, front view.
PDB entry 1p4u coloured by chain, side view.
PDB entry 1p4u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of accessory protein motifs by the gamma-adaptin ear domain of GGA3.
Miller GJ, Mattera R, Bonifacino JS, Hurley JH
Nat Struct Biol 10 599-606 (2003)
PMID: 12858162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-172242 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor-binding protein GGA3 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZ52 (Residues: 571-723; Coverage: 21%)
Gene names: GGA3, KIAA0154
Sequence domains: Adaptin C-terminal domain
Structure domains: Immunoglobulin-like
Rab GTPase-binding effector protein 1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15276 (Residues: 435-447; Coverage: 2%)
Gene names: RAB5EP, RABEP1, RABPT5, RABPT5A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 45.2Å b: 85.2Å c: 97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.255
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

11 review citations

Adaptors for clathrin coats: structure and function.
Owen et al. (2004)
10 more

2 mentions without citation

Structure and activation mechanism of the BBSome membrane protein trafficking complex.
Singh et al. (2020)
1 more